Advances in Life Science and Biotechnology

Evaluating metabolite profiling of endophytic contagious disconnects of five ethno-pharmacologically significant plants of Meghalaya


Adicath A. A and Abel W. O

Crude metabolite extracts derived from the culture broths of endophytic fungi of five ethnopharmacologically important plant species used by the ethnic tribes of the hill state of Meghalaya in North-East India were screened for the presence of toxins and secondary metabolites using agar plug paper chromatography, thin layer chromatography (TLC) and liquid chromatography-mass spectroscopy (LC-MS). The endophytic fungal isolates were found to produce a wide range of toxins, growth hormones and antibiotics upon searching in a fungal mycotoxin database. Relatedness among the fungal isolates was determined based upon their extra-cellular metabolite production pattern. The endophytic fungal isolates in the present study showed a wide range of metabolomic diversity and can be explored as potential microbial cell factories for production of a diverse range of biomolecules. Taxonomic relatedness of the endophytes based upon the snapshots of their secretomes, presented a contrasting picture in relation to their morphological identity. Rapid metabolome analysis of endophytic fungi using analytical techniques like LC-MS coupled with mass related search in fungal specific metabolite databases is bound to increase our insights about fungal chemo-taxonomy as a whole and bio-prospection of useful fungal metabolites in particular.


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