Advances in Animal Science, Theriogenology, Genetics and Breeding

Expeditious detection of Salmonella species in newborn calves by polymerase chain reaction


DF Moussa, KH Aaliyah, LS Mohammedu, SGVAmmedand AA Al-Doss

Fecal samples collected from 85 diarrheic calves and 65 apparently healthy contact calves were examined for the presence of Salmonellaspecies using bacteriological examination and fimAgene amplification assay (PCR). Salmonellawere isolated from 43.53% of diarrheic calves and from 27.69% of apparently healthy contact calves. Salmonella typhimuriumwere isolated from diarrheic and contact calves in percentages of 17.65 and 15.38% respectively; whereas Salmonella entertidiswere isolated in percentages of 11.76 and 7.69%, respectively. Salmonella dublinwere isolated from the diarrheic calves in a percentage of (8.24%) and contact calves in a percentage of (4.62%), but Salmonella anatumwere isolated from diarrheic calves only in a percentage of (5.88%), The sensitivity and specificity of PCR were 100 and 95.8%, respectively during examination of fecal samples obtained from diarrheic calves, while during the examination of those obtained from contact calves the percentages were 100and 89.4%, respectively.


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