Advance Journal of Virology, Epidemic and Pandemic Diseases

Molecular characterization of adenovirus causing acute respiratory diseases in Malaysia from 2003 to 2011


Mohd Apandi Y, Zarina M. Z., Khairul Azuan O., Nur Ismawati A. R. and Zainah S

Human Adenoviruses (HAdVs) are human pathogens that normally are associated with acute respiratory
disease (ARD). Here, in this study, we characterized human adenovirus isolated from respiratory
specimens collected from 2003 to 2011. Positive adenovirus were confirmed by cell culture and indirect
immunoflourescence (IF) techniques. The isolates and positive clinical samples were subjected to
adenovirus polymerase chain reaction, followed by DNA sequencing. BLAST searches and phylogenetic
analysis revealed that only 2 species of HAdV, species C at 64% (47/73) and species B at 36% (26/73)
were found circulating amongst ARD cases in Malaysia. Four types of HAdV species C were detected
namely: HAdV-2 (66%), HAdV-1(17%), HAdV-5(13%) and HAdV-6(4%). For HAdV species B, only 2 types
were detected with HAdV-3 being the highest at 58% and HAdV-7 at 42%. HadV-7 was associated with
severe infections.


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