Advances in Life Science and Biotechnology

Commentary - (2022) Volume 10, Issue 3

Methodological challenges in realising the potential of hybridization
Rica Mishra*
Department of Biotechnology, Sun Yat-sen University, Guangzhou, China
*Correspondence: Rica Mishra, Department of Biotechnology, Sun Yat-sen University, Guangzhou, China,

Received: 05-Aug-2022, Manuscript No. ALSB-22-77241; Editor assigned: 08-Aug-2022, Pre QC No. ALSB-22-77241 (PQ); Reviewed: 23-Aug-2022, QC No. ALSB-22-77241; Revised: 30-Aug-2022, Manuscript No. ALSB-22-77241 (R); Published: 07-Sep-2022, DOI: 10.51268/2736-1837.22.10.073


The geographical, temporal, demographic, and genomic complexity of hybridization in most biological systems where it occurs is highlighted by its role in facilitating or preventing speciation. One major challenge for researchers studying hybridization and its evolutionary consequences is that biological complexity is frequently overlooked in the analytical methods used to analyse genetic data and draw conclusions about hybridization's consequences. They highlight a few areas where current analytical methods fall short and emphasise how improvements to their analytical toolkit are required to make more reliable inferences about hybridization and speciation. Many hybridization studies aim to determine the extent to which hybridization results in genetic material introgression between populations or incipient species. The ability to precisely measure gene flow amounts and timing, as well as variation in gene flow across the genome, is critical for making inferences about a variety of phenomena, including the circumstances under which hybridization may facilitate or inhibit speciation, the time frame over which hybridising species have been in contact, the genomic architecture of reproductive isolation and divergent adaptation, and the likelihood and consequences of reinforcement. Current methodologies for measuring gene flow amounts and timing, on the other hand, generally rely on models that are not biologically realistic; in many cases, it is unclear what effect model violations may have on gene flow estimates, and in some cases, model violations are known to result in biased estimates. They briefly outline three issues where methodological advances would greatly benefit the study of hybridization and speciation. They estimating the timing of gene flow, (ii) distinguishing between primary and secondary contact and (iii) modelling the demographic conditions that allow for hybridization and gene flow.

For a variety of reasons, information on gene flow timing is of great interest to evolutionary biologists. For example, demonstrating gene flow during initial divergence could help to clarify the mode of speciation, including the possibility of sympatric speciation. Furthermore, it may be possible to understand the order in which different isolating barriers or divergent adaptations evolved by comparing the timing of gene flow at different genes or genomic regions. However, there is currently no reliable method for measuring the variation in gene flow rate over time. Recently, the most widely used method has been implemented in the Idiopathic Myelofibrosis (IM) suite of programmes and has been used to make inferences about the likelihood of sympatric speciation and the history of contact between species. It has been demonstrated, however, that it is uninformative, and a more complex model than that implemented in the IM suite is likely required, with additional parameters for migration rates at different time periods and for the time at which migration rates change.


However, the computational power required dealing with more complex models and larger data sets will increase significantly, and further research into the most efficient way to use genetic data would be beneficial. Furthermore, which (and how many) summary statistics to include will be determined by both the method of data collection and the method of analysis, and will necessitate additional research as these fields advance.

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